ORA™ qPCR Probe Mix, 2X

£106.00£477.00

highQu ORA™ qPCR Probe Mix is based on the small molecular inhibitor technology Hot Start PCR, which achieves extremely high sensitivity and specificity under both standard and fast qPCR cycling conditions. 

They provide excellent results on both AT and GC rich templates, in multiplexing and guaranty rapid extension with early Ct values with minimum or no optimisation.

Supplied with PCR Water to guarantee optimal performance.

To suit the broad instrument range the ORA qPCE Probe Master Mixes are available in three versions – without ROX, with low or high ROX concentration.

 

 

£106.00
£477.00
£477.00
£106.00
£106.00
£477.00

Description

highQu ORA™ qPCR Probe Mix, 2X

Applications

  • qPCR assays based on specific probes: TaqMan®, Molecular Beacons, Scorpions™ Probes
  • Gene expression analysis, quantification of: gDNA, cDNA, viral DNA, low copy number genes

Benefits

  • Universal – both standard and fast cycling, all probe qPCR assays
  • qPCR of GC or AT rich templates, single-plex & multiplexing
  • Rapid extension, early Ct
  • Supplied with PCR Water

Protocol

Important Notes

  • Use special primer selection programs for good planning.
  • Work with amplicons in a range of 80-200, max 400 bp.
  • Take typical measures to prevent PCR cross over contamination, keep your bench clean, wear gloves, use sterile tubes and filter pipet tips.
  • Run reactions in triplets; include a no-template control and positive control in parallel.
  • Thaw and keep reagents on ice. Mix well before use.

 

Prepare a 20 µl reaction

Reverse Primer 100 – 400 nM final c.

Forward Primer 100 – 400 nM final c.

Specific Probe 200 nM final c.

cDNA Template or  gDNA Template <100 ng or <1 μg

PCR Water to 10 μl

ORA™ qPCR Mix, 2X10 μl

  • Mix gently, avoid bubbles.
  • Place into the instrument set like:

Initial denaturation 1 cycle: 95°C – 2 min for cDNA  or
1 cycle: 95°C – 3 min for gDNA

Denaturation 40 cycles: 95°C – 5 sec

Anneal./extension 40 cycles: 60-65°C – 20-30 sec

  • Follow instrument instructions for melt curve analysis