HotShot Sapphire PCR Master Mix

£225.00

  • 5 x 1ml
  • Hot Start Ab Mediated Taq
  • Works from the very first cycle
  • Exceptional buffers providing excellent PCR conditions
  • Blue Loading dye for ease of transfer to gel
  • Designed to work with complex, multiplex PCR

Product Code: HS003

SKU: SQ9008132 Categories: , Product ID: 395

Description

Features & benefits

  • All of our PCR Master Mixes go through stringent QC/QA procedures to ensure batch to batch performance
  • Our PCR Master Mix is designed to amplify DNA irrespective of the source with the use of a thermocycler
  • The Taq polymerase used in our PCR Master Mix is inactive until the PCR Master Mix has been incubated at 95C for five minutes thus ensuring greater specificity
  • They contain everything you need for PCR except DNA and Primers
  • We’ve optimised a starting MgCl2 concentration of 6mM and alongside our Taq polymerase we’ve added our proprietary PCR buffers to ensure that you get required results from the start
  • They work with 3 step, 2 step and touchdown PCR protocols
  • We supply our PCR Master Mix in a 2x concentration for convenience (for set up of both small & large volume reactions)
  • They can be used for amplifying sequences with sizes below 100bp and up to at least 1.5kb

High Resolution Melting

  • HotShot Diamond PCR Master Mix works very well for High Resolution Melting with LCGreen Plus™  dye (BioFire Inc). LCGreen Plus dye has been shown to be the best dye for High Resolution Melting techniques.
  • Is our HotShot PCR Master Mix a mix for all the seasons? Perhaps, but we also offer the SureShot PCR Master mix for routine PCR aimed at gel electrophoresis.
  • HotShot Sapphire PCR Master Mix for those multiplex assays that are going straight to gel. We’ve added an inert blue dye to our Sapphire for you.