highQu Dye Based 1 Step RT-QPCR Kits



  • Dye-based RT-qPCR on instruments requiring either high or low ROX concentration.
  • Relative gene expression analysis
  • Quantification of mRNA, total RNA, viral RNA, low copy number genes


  • Both standard and fast cycling, GC rich templates
  • Rapid extension, early Ct
  • Supplied with PCR Water


highQu 1Step RT qPCR mastermixes in combination with a blend of thermostable and extremely active Reverse Transcriptase & advanced RNase Inhibitor (RT Mix) allow for a single step one tube RT qPCR.

qPCR mastermixes are based on the small molecular inhibitor technology Hot Start PCR allowing to achieve highest sensitivity and specificity under both standard and fast qPCR cycling conditions. They provide excellent results on both AT/GC rich templates and guaranty rapid extension with early Ct values with minimum or no optimization.

Our mastermixes are supplied with PCR Water to guaranty the best performance. To suit the broad instrument range the 1Step RT qPCR Green Mixes are available in two versions – with low or high ROX concentration.