high resolution melting

Identify variations in nucleic acid sequences post PCR quickly, easily and cheaply using High Resolution Melting. The below products have been chosen to speed up and facilitate HRM analysis, enabling the researcher to detect epigenetic differences, polymorphisms and mutations in double stranded DNA samples.

hrm dye button

Products from BioFire Defense specifically designed for High Resolution Melting and for use with their LightScanner Instrument.

BioFire Defense

Tailored specifically for HRM. Extremely stable and does not inhibit PCR. Detects heteroduplexes during melting analysis after PCR, simply add the dye to your sample before PCR.

Ready to use PCR master mix with LCGreen Plus Dye. Provides a pre-optimised solution to deliver superior PCR performance for Hi-Res Melting®and genotyping applications

PCR Mastermix products

We offer a variety of widely used PCR mastermix products from HighQu and Microzone as well as our own range.

highqu logo

A classic enzyme for routine PCR applications

Perfect for complex/longer templates and fast cycling

“Robust, proofreading, hot start”
Effective for low copy detection, long or high fidelity PCR, amplification of complex templates, crude sample PCR and multiplexing

For demanding PCR applications such as amplification of complex templates, crude sample PCR and fast cycling

With 50 x higher fidelity than taq polymerase

microzone logo

Ready-to-use PCR master mix containing recombinant Taq polymerase

A PCR master mix with dye that has all the advantages of MegaMix with the addition of a blue agarose loading dye

Elite hot start PCR mastermix designed for challenging PCR systems, supplied double concentrated

Elite hot start mastermix: A recalibration of Megamix Royal with the same dye as Megamix Blue, supplied double concentrated

clent life science logo

Used for many years with LCGreen Plus dye as a convenient and effective kit for HRM

Hot start, Ab mediated Taq with blue agarose loading dye for ease of transfer to gel

Exceptional amplification for everyday applications

LightScanner® System

The LightScanner, having been discontinued, is no longer available for purchase.

Please feel free to contact us to discuss further.

Utilised in Mutation Discovery, Gene Scanning and Genotyping, the LightScanner® (from Biofire Defense) was the first system to use Hi-Res Melting® (also known as High Resolution Melting, or HRM) analysis for mutation scanning and genotyping in a 96 or 384 well plate system.