Identify variations in nucleic acid sequences post PCR quickly, easily and cheaply using High Resolution Melting. The below products have been chosen to speed up and facilitate HRM analysis, enabling the researcher to detect epigenetic differences, polymorphisms and mutations in double stranded DNA samples.
High Resolution Melting Dye
Products from BioFire Defense specifically designed for High Resolution Melting and for use with their LightScanner Instrument.
Tailored specifically for HRM. Extremely stable and does not inhibit PCR. Detects heteroduplexes during melting analysis after PCR, simply add the dye to your sample before PCR.
Ready to use PCR master mix with LCGreen Plus Dye. Provides a pre-optimised solution to deliver superior PCR performance for Hi-Res Melting®and genotyping applications
PCR Mastermix Products
We offer a variety of widely used PCR mastermix products from HighQu and Microzone as well as our own range.
A classic enzyme for routine PCR applications
Perfect for complex/longer templates and fast cycling
“Robust, proofreading, hot start”
Effective for low copy detection, long or high fidelity PCR, amplification of complex templates, crude sample PCR and multiplexing
For demanding PCR applications such as amplification of complex templates, crude sample PCR and fast cycling
With 50 x higher fidelity than taq polymerase
Ready-to-use PCR master mix containing recombinant Taq polymerase
A PCR master mix with dye that has all the advantages of MegaMix with the addition of a blue agarose loading dye
Elite hot start PCR mastermix designed for challenging PCR systems, supplied double concentrated
Elite hot start mastermix: A recalibration of Megamix Royal with the same dye as Megamix Blue, supplied double concentrated
Hot start, Ab mediated Taq with blue agarose loading dye for ease of transfer to gel
Exceptional amplification for everyday applications
The LightScanner, having been discontinued, is no longer available for purchase.
Please feel free to contact us to discuss further.
Utilised in Mutation Discovery, Gene Scanning and Genotyping, the LightScanner® (from Biofire Defense) was the first system to use Hi-Res Melting® (also known as High Resolution Melting, or HRM) analysis for mutation scanning and genotyping in a 96 or 384 well plate system.