high resolution melting

Identify variations in nucleic acid sequences post PCR quickly, easily and cheaply using High Resolution Melting. The below products have been chosen to speed up and facilitate HRM analysis, enabling the researcher to detect epigenetic differences, polymorphisms and mutations in double stranded DNA samples.

High Resolution Melting Dye

Products from BioFire Defense specifically designed for High Resolution Melting and for use with their LightScanner Instrument

 BioFire Defense
LC Green Plus Tailored specifically for HRM. Extremely stable and does not inhibit PCR. Detects heteroduplexes during melting analysis after PCR, simply add the dye to your sample before PCR
LightScanner Mastermix Ready to use PCR master mix with LCGreen Plus Dye. Provides a pre-optimised solution to deliver superior PCR performance for Hi-Res Melting®and genotyping applications

PCR Mastermix Products

We offer a variety of widely used PCR mastermix products from HighQu and Microzone as well as our own range.

Taq DNA Polymerase A classic enzyme for routine PCR applications
ALLin™ Hot Start Taq Polymerase Perfect for complex/longer templates and fast cycling
ALLin™ RPH Polymerase “Robust, proofreading, hot start”
Effective for low copy detection, long or high fidelity PCR, amplification of complex templates, crude sample PCR and multiplexing
ALLin™ Taq DNA Polymerase For demanding PCR applications such as amplification of complex templates, crude sample PCR and fast cycling
ALLin™ Hifi DNA Polymerase With 50 x higher fidelity than taq polymerase

Megamix & Megamix Double Ready-to-use PCR master mix containing recombinant Taq polymerase
MegaMix Blue & MegaMix Blue Double A PCR master mix with dye that has all the advantages of MegaMix with the addition of a blue agarose loading dye
Megamix Gold Elite hot start PCR mastermix designed for challenging PCR systems, supplied double concentrated
Megamix Royal Elite hot start mastermix: A recalibration of Megamix Royal with the same dye as Megamix Blue, supplied double concentrated

  clent life science logo
HotShot Diamond Used for many years with LCGreen Plus dye as a convenient and effective kit for HRM
HotShot Sapphire Hot start, Ab mediated Taq with blue agarose loading dye for ease of transfer to gel
SureShot Exceptional amplification for everyday applications

LightScanner® System

lightscanner instrument

The LightScanner, having been discontinued, is no longer available for purchase.

Please feel free to contact us to discuss further.

Utilised in Mutation Discovery, Gene Scanning and Genotyping, the LightScanner® (from Biofire Defense) was the first system to use Hi-Res Melting® (also known as High Resolution Melting, or HRM) analysis for mutation scanning and genotyping in a 96 or 384 well plate system.