For many reasons, genotyping has settled into our everyday lives. It provides information for our medical records, scientific research and even for antibiotic resistance. But first, what is Genotyping? Genotyping is a technology that determines an individual or animal’s genotype. Genotypes are usually small genetic differences which could cause important physical changes. For example, in … Read more
With polymerase chain reaction (PCR), scientists are able to harness the power of the genome. The PCR process is an essential tool in biology because it allows an ample supply of DNA segments to be generated from relatively small samples. Although PCR reactions can be finicky and unpredictable, the use of a PCR master mix … Read more
Taq polymerase is one of the most ubiquitous enzymes in molecular biology. Since its discovery in 19651, Taq has become the backbone of PCR and all the downstream applications that technology enables. This powerful polymerase is the default choice for most amplification and cloning procedures, as well as diagnostic tests, marker gene DNA sequencing, and … Read more
Antibiotics are among the most important discoveries of the 20th century, changing the way that the world approaches medicine, food safety, and agriculture. But most researchers are all too aware that the prevalence of bacterial strains that are resistant to one or more common antibiotics is on the rise. In addition, new research suggests that … Read more
Taq polymerase is the tried and true standard when it comes to PCR, but that doesn’t mean it’s the most accurate polymerase for high-sensitivity applications. Instead, alternative enzymes, known as high-fidelity polymerases, offer higher accuracies in the amplicons resulting from PCR thanks to a combination of more stringent base reading and proofreading activities. While you … Read more
We’re no strangers to PCR (Polymerase Chain Reaction) here at Clent Life Science and if you’re reading this, chances are you aren’t either. The enzymatic assay is used to create multiple copies of a specific segment of DNA from a complex combination of DNA molecules. It’s still celebrated as one of the most important developments … Read more