High Resolution Melting

Mutations in PCR products are detected by the presence of heteroduplexes, optimally through use of our LCGreen® Plus dye. Variations are identified by changes in the shape of the melting profile when compared to a reference sample. Hi-Res Melting® (HRM)  was used on the LightScanner® for mutation discovery in tumor samples. The assays provided information on mutations present in low allele fractions (as low as 5%) and allelic imbalances between tumor and matched normal samples.