HotShot Diamond PCR MasterMix
HS002 (5x1mL)
HotShot Diamond™ PCR Master Mix Overview:
Our PCR Master Mix is designed to amplify DNA irrespective of the source with the use of a Thermocycler. The Taq polymerase used in our PCR Master Mix is inactive until the PCR Master Mix has been incubated at 95C for five minutes thus ensuring greater specificity. HostShot Diamond PCR Master Mix (as with all of our PCR Master Mixes) contain everything you need for PCR expect for your DNA and Primers. Furthermore, our PCR Master Mix works with 3 step, 2 step and touchdown PCR protocols.
We supply our PCR Master Mix in a 2x concentration for convenience to be able to set up of both small & large volume reactions. Our PCR Master Mix can be used for amplifying sequences with sizes below 100bp and up to at least 1.5kb.
The Extra Kick:
The first few cycles of PCR are always the most important. Our PCR Master Mix has been developed to provide specific, robust amplification of your DNA from the first cycle! We’ve optimised a starting MgCl2 concentration of 6mM anf Taq polymerase and added our proprietary PCR buffers to ensure that you get the results required from the start. All of our PCR Master Mixes go through stringent QC/QA procedures to ensure batch to batch performance.
Stability:
We know that the stability of your PCR Master Mix is important. That is why we’ve continually assessed and refined our buffers to ensure that our PCR Master Mix can be repeatedly freeze thawed without loss of activity of the Taq polymerase or breakdown of the buffers required to ensure that the PCR Master Mix performs to your requirements.
Workflow:
PCR has become the backbone of many a lab using molecular biology techniques. Our PCR Master Mix comes in one tube, requires very little pipetting and is therefore fast to set up. With their superior stability, our PCR Master Mixes can be automated using robotic systems.
High Resolution Melting:
Finally, we know that the HotShot Diamond PCR Master Mix works very well for High Resolution Melting with LCGreen Plus™ dye(Idaho Technologies Inc). LCGreen Plus dye has been shown to be the best dye for High Resolution Melting techniques.
Is our HotShot PCR Master Mix a mix for all the seasons? Perhaps, but we also offer SureShot PCR Mastermix for routine PCR aimed at gel electrophoresis. We also offer HotShot Sapphire PCR Master Mix for those multiplex assays that are going straight to gel. In our Sapphire we've added an inert blue dye.
We're proud of our PCR Master Mix if you would care to try before you buy then please contact us, as samples are available.
The PCR process is covered by patents issued and applicable in certain countries. Clent Life Science does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license.